"You can go to Jolly Pirate Donuts and take a two-hour SHIT for all I care!"."The garbage disposal sounds like Chewbacca taking a shit!".Wheel of Fortune (Ever since he was refused as a contestant, he no longer gives a shit about it and wants to call Vanna White a bitch in person and tie Pat Sajak to a telephone pole and let the UPS guy buttfuck him).Full House (He still hates Bob Saget though). ![]() Jeopardy (He once had a dream where Alex Trebek had a picture of a giraffe in his ass during an earthquake).Previous Video | Next Video Favorite Songs Once was refused as a contestant on Wheel of Fortune and said he wanted to call Vanna White a bitch in person and tie Pat Sajak to a telephone pole and let the UPS guy buttfuck him.Then the manager called him a DUMBASS, like he's his DAD and SHIT. Once kicked out of a Lens Crafters because he couldn't stop calling people DICKS.Can silence a door-to-door salesman by telling him to make like a banana and SHIT!.He is unaware of the location of his GOD DAMN pants.Also, WHAT THE FUCKING PISS, THAT WAS FUCKING STUPID. Once massaged Heads & Shoulders Shampoo into his eyes just for the SHIT COCK of it.Drives an ass-fuck ugly red '94 Geo Prizm. ![]()
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Guitar – Playing guitar for over 5 years, performing at open mics in local venues.ġ What’s the Best Format for a College Freshman Resume?.Web Designing – Creating visually appealing web designs through HTML5/CSS3 scripting.Served as mentor to two new members in the association.Member of the Association for Computing Machinery (ACM) since 2020. Fifth Place in Programming Competition - UPRM (2019).Certified Coding Bootcamp Graduate – CodeLocal Academy (2020).Web Development-Django Framework, HTML5/CSS3.Programming Languages-Python, Java, SQL.Coded a website for an Artificial Intelligence course that was used in the following semester.Participated in a hackathon for mobile application development and won second place.Recipient of a full National Science Foundation Research grant in recognition of research proposal in the field of artificial intelligence.Member of the Association for Computing Machinery (ACM) since 2020.Relevant Extracurricular Activities & Academic Achievements University of Puerto Rico - Mayaguez Campus, PR Successfully created an interactive UI for customer-facing applications that increased user engagement by 20%.Developed detailed documentation for each project throughout the code development process.Designed visuals for websites using Adobe Photoshop software applications.Assisted software development team with backend coding tasks using SQLite database management system.Created intuitive user interfaces (UI) for customer-facing applications using HTML5/CSS3 standards.Developed and tested web applications utilizing Django framework and Python language.Looking forward to bringing my passionate enthusiasm for software development, critical thinking abilities, and growth mindset to the role of Junior Web Developer at SoftDev. Possesses strong technical aptitude, excellent communication skills, and demonstrated history of academic excellence. See 20+ resume templates and create your resume here.īachelor of Science in Computer and data-driven college freshman pursuing a Bachelor of Science in Computer Science from the University of Puerto Rico Mayaguez Campus with the desire to contribute my knowledge and skills to Matrix Systems as a Junior Software Developer. ![]() Plus, you’ll get ready-made content to add with one click. Want to save time and have your resume ready in 5 minutes? Try our resume builder. How to describe your experience on a resume for a college freshman to get any job you want. ![]() Tips and examples of how to put skills and achievements on a college freshman resume.How to write a college freshman resume that will land you more interviews.A college freshman resume example better than 9 out of 10 other resumes.With our advice, you won't end up underpaid and working under Michael Scott. Out of the frying pan and into the fire.īut don't worry, a great college freshman resume will do the trick. ![]() Maybe to reduce your student debt, or just to be able to socialize outside the dining hall. It's no longer just about making class and keeping up with that annoyingly smart 14-year-old classmate. Barely started college, and here you are-entering the job market. These include: their use of the best quality materials and hardware custom-tailored designs contemporary designs upfront, transparent, and competitive pricing and respect for the client’s home, which means they make sure to clean up after completing the project. They take pride in a number of distinctive factors that differentiate them from similar providers in the Sunshine Coast Region. The company has established a reputation as a provider of top-quality pool fencing and balustrades and they are ready to work with property owners to fully understand their needs and expectations. 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Ivanka Trump was the last witness called before the attorney general’s office rested its case. She followed testimony from her father and her brothers Donald Jr. The trial has spanned nearly six weeks so far with testimony from a total of 25 witnesses – 24 of which were in person. ![]() The NY attorney general’s office has rested its civil case against former President Donald Trump, his adult sons Donald Jr. “And despite the fact that she was very, very nice, very friendly, the facts, basically, demonstrate the truth.” ![]() And clearly you cannot distance yourself from that fact. “At the end of the day, this case is about fraudulent statements of financial condition that she benefitted from, she was enriched. Trump and her brothers and for herself,” James said. “The reality is, is that based on the evidence, the documentary evidence, she clearly was involved in negotiating and securing loans, favorable loans, for the benefit of the Trump Organization, for Mr. “Ivanka Trump was cordial, she was disciplined, she was controlled and she was very courteous but her testimony raises questions with regards to its credibility, which will be a question for the finding of fact,” New York Attorney General Letitia James said outside court. Ivanka Trump was “cordial” and “very courteous,” but the New York attorney general questioned the credibility of her testimony Wednesday. New York Attorney General Letitia James departs from the New York State Supreme Court following Wednesday's proceedings. The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition. Tested in polyacrylamide gel electrophoresis and Western Blotting. One prominent feature of this protein ladder, is that it remains in the liquid state at the storage temperature therefore, there is not a freeze-thaw cycle involved, making the ladder very stable.Ġ.1~0.4 mg/ml of each protein in 20 mM Trisphosphate (pH 7.5 at 25☌), 2% SDS, 1 mM reducing reagent, 3.6 M Urea, and stabilizer.įor each application, 5-15µl is needed, depending on the size of the wells. No need to heat, dilute, or add reducing agent before loading. The ladder is supplied in protein gel loading buffer and is ready-to-use. Two orange reference bands at 70 kDa and 25 kDa and one green reference band at 10 kDa highlight the blue-stained protein ladder. It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for sizing of proteins. The PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins (10 to 250 kDa) for use in protein electrophoresis (SDS-PAGE) and western blotting. The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. During electrophoresis, the polypeptides of the ladder show up in the gel as distinctly colored bands. What this means is that before even loading the ladders into the gel for electrophoresis, these ladders are previously stained and ready to go. Western Blotting Reagents 2.4 NF-kB Reporter Assay Using RAW-Blue Cells. In a prestained protein ladder, each of these bands, or polypeptides, are already stained with a dye. Use 5-15µl, depending on the size of the wellsĪpplication: The Bullseye Pre-stained Protein Ladder is suitable for visualizing proteins during electrophoresis without staining and for monitoring transfer onto membrane. protein concentrations were determined using Pierce BCA protein analysis kit.2 reference bands - red at ~75kDa and green at ~25kDa.Load ladder into SDS-PAGE gel or Western blot using these volumes (based on gel thickness of 0.75 1.0mm). Mix solution gently to ensure that it is homogeneous. Stoolfix Gut Microbiome Stabilization Kitsīullseye Pre-stained Protein Ladder, 10-180 kDa mPAGE ® Western Protein Standards consist of seven recombinant proteins at 20 kDa, 30 kDa, 40 kDa, 50 kDa, 60 kDa, 80 kDa and 120 kDa with an IgG binding site that binds to most antibodies. Thaw ladder at room temperature to dissolve precipitated solids.Beakers, Graduated Cylinders, and Flasks.Aluminum Foil, Parafilm, and Multiwipes 5l of IRIS11 Prestained Protein Ladder (PMI11) resolves 11 bands in 4-20 SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.Thaw the ladder either at room temperature or at 37-40☌ for a few minutes to dissolve precipitated solids. The quality of the BLUeye Prestained Protein Ladder is tested on a lot-to-lot basis to ensure consistent product quality.īLUeye Prestained Protein Ladder Protocolġ. ![]() Sizing of proteins on SDS-PAGE and Western blots.Īpproximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Trisphosphate, pH 7.5 at 25☌), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol. Monitoring of protein transfer onto membranes during Western blots. ![]() Monitoring of protein migration during SDS-PAGE. These protein standards and ladders are consistent from lot to lot and are strictly. Each protein standard or ladder is supplied in a ready-to-use format, eliminating the need to reduce, pre-mix, or add loading dyes. Ready-to-use: supplied in a loading buffer for direct loading on gelsĮasy to identify: includes the ~25, ~75 kDa reference bands coupled with a green and a red dye Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications. Stable at -20C for 2 years Stable at 4C for 6 months. The ladder is supplied in gel loading buffer and is ready to use.īroad range: 10-245 kDa (Tris-glycine-SDS running buffer) Quality Control Tested in SDS-polyacrylamide gel electrophoresis and western blotting. The BLUeye Prestained Protein Ladder is designed for monitoring protein separation during, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with Tris-glycine-SDS running buffer. The BLUeye Prestained Protein Ladder is a three-color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 to 245 kilodalton (kDa). The Angle Bisector Theorem is a mathematical principle that states that a point on a bisector is equidistant from each of the angles it cuts. You can also use a sharp pencil or a pencil with a small compasses to get an accurate result. However, they must be clearly visible in your final answer. ![]() You should draw the arcs for a perpendicular bisector lightly to ensure accuracy. They are a great resource for students in fifth and eighth grades. These worksheets help students learn how to find the angle bisector of a triangle. There is also space for a teacher's answer page, so students can review their answers. Its main focus is to teach students the interior and exterior angles of triangles. The Angle Triangle Worksheet can be used for both basic and advanced mathematics. The worksheet features sample questions, too. This worksheet can be used by students to calculate the sum of interior triangle angles. The other two angles are represented by an algebraic expression, such as x + 5. It is composed of 12 different triangles, each with a given angle. This Angle Triangle Worksheet teaches students how to measure angles. You can use the search bar to locate the worksheet you are looking for if you aren't sure. In addition, we'll talk about Isosceles and Equilateral triangles. Grade 5 Angles And Triangles Worksheets - This article will discuss Angle Triangle Worksheets as well as the Angle Bisector Theorem. Find what you need about Grade 5 Angles And Triangles Worksheets down below. ![]() Many of these problems take more than one or two steps, so look at it as a puzzle and put your pieces together!īelow you can download some free math worksheets and practice.If you are trying to find Grade 5 Angles And Triangles Worksheets, you are arriving at the right site. If you don’t remember that last step, don’t worry! You can just take two more steps and find the 3 rd angle of the bottom triangle and subtract it from 180°to find the exterior angle. ![]() We need a few pieces of the puzzle before we can find the measure of x. They ultimately want to find the measure of that exterior angle. There’s actually at least three different ways that you can answer this problem. Find a piece at a time and put them together until you reach your answer! You have to look at these problems as “puzzles” because sometimes you need to find a part that they are not asking for in order to find the final result. Let’s see if we can put these properties to work and answer a few questions. So, in EVERY equilateral triangle, the angles are always 60°. This is because all angles in a triangle always add up to 180°and if you divide this amongst three angles, they have to each equal 60°. The angles, however, HAVE to all equal 60°. The sides can measure anything as long as they are all the same. When all angles are congruent, it is called equiangular. ![]() In an equilateral triangle, all sides are congruent AND all angles are congruent. Here are some diagrams that usually help with understanding. Since two sides are congruent, it also means that the two angles opposite those sides are congruent. Well, some of these types of triangles have special properties!Īn isosceles triangle has two sides that are congruent. We’ve learned that you can classify triangles in different ways. 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There's nothing worse than that curious sensation that your opponent isn't quite human. Tons of different creators with tons of different styles. In addition to those, YouTube vids are the way to go for learning about chess. Have your kids play and learn chess online with the best tools- tactics, puzzles, videos, lessons & more. Simply press "Z" to enter Focus Mode in your game. and are the best two sites to play and learn on. ![]() With tournaments of different forms and time controls starting every few minutes, there is always a place to compete.ĭon't want any distractions while you play? Use our popular Focus Mode to get down to what matters: you, your opponent, a board, and a clock. Want to raise the stakes? Try a tournament! Our free tournaments allow you to pit your skills against a field of players, battling to emerge atop the leaderboard. 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Who doesnt want to be a cool intellectual and play chess like a pro This game definitely stands out from everything else and has tons of. Our Play server has all the features you could ever want:Ĭ has by far the most chess players online at any moment. Are you looking for somewhere to enjoy a nice game of chess? Maybe you’ve asked yourself: What is the best place to play chess online?Ĭ is the best place to play chess online. |
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